Journal: BMC Microbiology

Article Title: First human cell-based cultivation system for the syphilis spirochete Treponema pallidum

doi: 10.1186/s12866-026-04856-5

Figure Lengend Snippet: Human foreskin fibroblast cell lines support the growth of T. pallidum in vitro . A Human foreskin fibroblast cell lines (MoNa, HFF1, and HFFC) support the growth of T. pallidum in long-term, continuous, in vitro cultivation. While cultivation of strain SS14 was discontinued after 10 weeks, in vitro cultures of the DAL-1 strain have been ongoing for over one year. During this period, scheme of subcultures was optimized (up to 1:20). A booster passage (*), extending the standard 7-day subculture to a 14-day period with a cultivation medium exchange on day 7, was employed to enrich the treponemal culture during critical declines. In vitro cultivation was performed in triplicate (i.e., in three cultivation wells) and the mean values for each subculture are presented in graph. Source data from individual wells are shown in Supplementary Table . B Parallel cultivation for seven days ( n = 15) with defined DAL-1 inoculum (10 6 treponemes) validated the differences in growth support of individual foreskin cell lines. Treponemal growth on human foreskin fibroblast cells was slower compared to growth on rabbit epithelial Sf1Ep cells. Red bar, mean. C Human foreskin-based cultivation system supports the growth of various T. pallidum strains ( n = 8), from the Nichols-like as well as the SS14-like cluster. Note that human foreskin fibroblast cells were prepared as an equal mixture of three tested cell lines. Each T. pallidum strain was cultivated in a single in vitro well, representing a sole experimental replicate used for data acquisition

Article Snippet: Human foreskin fibroblasts HFF1 (SCRC-1041; ATCC) and HFFC (300715; Cytion) were purchased, while the third cell line (MoNa) was kindly provided by Dr. Vladimir Rotrekl (Masaryk University), and was originally obtained from the National Tissue Centre (Czech Republic).

Techniques: In Vitro

Journal: Nutrients

Article Title: Essential Oils, Pituranthos chloranthus and Teucrium ramosissimum , Chemosensitize Resistant Human Uterine Sarcoma MES-SA/Dx5 Cells to Doxorubicin by Inducing Apoptosis and Targeting P-Glycoprotein

doi: 10.3390/nu13051719

Figure Lengend Snippet: Effects of Pituranthos chloranthus (PC) and Teucrium ramosissimum Desf. (TR) extracts on normal primary human uterine fibroblast cells (HUF) and primary murine Bone Marrow-Derived Macrophages (BMDM) viability. After treatment of primary HUF and murine BMDM with increasing concentrations (0–100 µg/mL) of PC and TR for 72 h, the percentage of viable cells was assessed using the (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. ( A ) Dose–response curves of PC-treated HUF (left panel) and TR-treated HUF (right panel). ( B ) Dose–response curves of PC-treated BMDM (left panel) and TR-treated BMDM (right panel). Data are expressed as a mean percentage of control growth ± Standard Deviation (SD) of two representative experiments ( n = 6 replicates per concentration).

Article Snippet: Primary human uterine fibroblast normal cells (HUF) were obtained from the ATCC.

Techniques: Derivative Assay, MTT Assay, Control, Standard Deviation, Concentration Assay

Journal: The Journal of investigative dermatology

Article Title: Wavelength-specific synergy between ultraviolet radiation and interleukin-1 alpha in the regulation of matrix-related genes: mechanistic role for tumor necrosis factor-alpha.

doi: 10.1046/j.1523-1747.1999.00681.x

Figure Lengend Snippet: Figure 1. Effects of specific wavelengths on the ability of IL-1α to stimulate matrix-related mRNA in fibroblasts. Human cultured fibroblasts were irradiated with 30 mJ of UVB, 5 J of UVA, 5 J of UVA1, or sham irradiated, and then given media without (minus symbols) or with (plus symbols) 10 ng IL-1α per ml, followed by 24 h of incubation at 37°C. Displayed are normalized levels of tropoelastin (A) and collagenase (B) mRNA.

Article Snippet: Cultured cells Normal human fibroblasts were obtained from the American Type Culture Collection (ATCC, Rockville, MD, catalog no. 1828X-CRL) and grown in Dulbecco’s modified Eagle’s medium supplemented with 10% fetal bovine serum.

Techniques: Cell Culture, Irradiation, Incubation

Journal: The Journal of investigative dermatology

Article Title: Wavelength-specific synergy between ultraviolet radiation and interleukin-1 alpha in the regulation of matrix-related genes: mechanistic role for tumor necrosis factor-alpha.

doi: 10.1046/j.1523-1747.1999.00681.x

Figure Lengend Snippet: Figure 2. Effects of specific wavelengths on cellular secretion of TNF-α. (a) Fibroblasts were irradiated with 30 mJ of UVB, 5 J of UVA, 5 J of UVA1, or sham irradiated, and then given media without (minus symbols) or with (plus symbols) 10 ng IL-1α per ml, followed by 24 h of incubation at 37°C. Displayed are TNF-α concentrations in the conditioned media (n 5 2). (b) Keratinocytes were similarly irradiated, incubated, and media assayed for TNF-α. Displayed are TNF-α concentrations in the conditioned media when no exogenous IL-1α had been added (n 5 2). Separate experiments demonstrated that exogenous IL-1α did not affect secretion of TNF-α by keratinocytes exposed to any of these wavelengths. (c) Neonatal and adult keratinocytes were irradiated with 10 or 30 mJ per cm2 of UVB or 5 or 20 J per cm2 of UVA. Displayed are TNF-α concentrations in the conditioned media from irradiated neonatal (n 5 2) and adult (n 5 3) keratinocytes, p , 0.01 for adult 30 mJ per cm2 UVB relative to sham by analysis of variance, then Dunnett q9 statistic.

Article Snippet: Cultured cells Normal human fibroblasts were obtained from the American Type Culture Collection (ATCC, Rockville, MD, catalog no. 1828X-CRL) and grown in Dulbecco’s modified Eagle’s medium supplemented with 10% fetal bovine serum.

Techniques: Irradiation, Incubation

Journal: The Journal of investigative dermatology

Article Title: Wavelength-specific synergy between ultraviolet radiation and interleukin-1 alpha in the regulation of matrix-related genes: mechanistic role for tumor necrosis factor-alpha.

doi: 10.1046/j.1523-1747.1999.00681.x

Figure Lengend Snippet: Figure 3. Effects of exogenous TNF-α on the ability of IL-1α to stimulate tropoelastin mRNA in fibroblasts. Human cultured fibroblasts were incubated with or without IL-1α and TNF-α, as indicated. Displayed are normalized tropoelastin mRNA levels after 24 h of incubation at 37°C. IL-1α produced a statistically significant increase in tropoelastin mRNA only in the cells that received no TNF-α (p , 0.01 by t test).

Article Snippet: Cultured cells Normal human fibroblasts were obtained from the American Type Culture Collection (ATCC, Rockville, MD, catalog no. 1828X-CRL) and grown in Dulbecco’s modified Eagle’s medium supplemented with 10% fetal bovine serum.

Techniques: Cell Culture, Incubation, Produced

Journal: The Journal of investigative dermatology

Article Title: Wavelength-specific synergy between ultraviolet radiation and interleukin-1 alpha in the regulation of matrix-related genes: mechanistic role for tumor necrosis factor-alpha.

doi: 10.1046/j.1523-1747.1999.00681.x

Figure Lengend Snippet: Figure 4. Effect of anti-TNF-α antibodies on the ability of UVB to block the effect of IL-1α on matrix-related messages. Human cultured fibroblasts were irradiated with 30 mJ of UVB or sham irradiation, and then given media without (minus symbols) or with (plus symbols) 10 ng IL-1α per ml, plus the indicated concentrations of anti-TNF-α inhibitory antibodies. Displayed are normalized tropoelastin (a) and collagen (b) mRNA levels after 24 h of incubation at 37°C.

Article Snippet: Cultured cells Normal human fibroblasts were obtained from the American Type Culture Collection (ATCC, Rockville, MD, catalog no. 1828X-CRL) and grown in Dulbecco’s modified Eagle’s medium supplemented with 10% fetal bovine serum.

Techniques: Blocking Assay, Cell Culture, Irradiation, Incubation

Journal: Pharmaceuticals

Article Title: Ethanolic Fenugreek Extract: Its Molecular Mechanisms against Skin Aging and the Enhanced Functions by Nanoencapsulation

doi: 10.3390/ph15020254

Figure Lengend Snippet: Anti-aging activities’ evaluation of fenugreek extract: ( a ) In vitro collagenase inhibition of fenugreek extract (denoted as Extract) and liponiosome encapsulating fenugreek extract (denoted as LNF). ( b ) Effect of fenugreek extract on collagen production. Human dermal fibroblast cells were treated with 125 µg/mL of fenugreek extract and rutin, and 50 µg/mL of vitamin C as a positive control and 0.005% DMSO as a control (vehicle), for 7 and 14 days. Data are reported as means ± SD ( n = 3). *, p < 0.05; ***, p < 0.001.

Article Snippet: Human dermal fibroblast cells were purchased from the American Type Culture Collection: ATCC (PCS-201-010) and immortalized human keratinocytes (HaCaT) were purchased from Cell Lines Service, Germany (Cat. No. 300493).

Techniques: In Vitro, Inhibition, Positive Control, Control

Journal: Pharmaceuticals

Article Title: Ethanolic Fenugreek Extract: Its Molecular Mechanisms against Skin Aging and the Enhanced Functions by Nanoencapsulation

doi: 10.3390/ph15020254

Figure Lengend Snippet: Evaluation of LNF activities: ( a ) Cytotoxicity induced by LNF: The effect of blank, LNF, and fenugreek extract on cell viability. Human dermal fibroblast cells were treated with different concentrations of blank (liponiosome without fenugreek extract denoted as Blank), LNF, and fenugreek extract for 24 h. Data are means ± SD ( n = 3). ( b ) Collagen production induced by LNF: The effect of LNF on collagen production. Human dermal fibroblast cells were treated with 0.005% DMSO as a control (vehicle), 7 µg/mL of extract, and 100 µg/mL of LNF (7 µg/mL of extract equivalence), together with blank particles, for 7 and 14 days. Data are represented as means ± SD ( n = 3). *, p < 0.05.

Article Snippet: Human dermal fibroblast cells were purchased from the American Type Culture Collection: ATCC (PCS-201-010) and immortalized human keratinocytes (HaCaT) were purchased from Cell Lines Service, Germany (Cat. No. 300493).

Techniques: Control

Journal: Pharmaceuticals

Article Title: Ethanolic Fenugreek Extract: Its Molecular Mechanisms against Skin Aging and the Enhanced Functions by Nanoencapsulation

doi: 10.3390/ph15020254

Figure Lengend Snippet: Inhibition of UV-induced MMPs and interleukin secretion on co-cultured skin cells by fenugreek extract and LNF: ( a ) Effect of UV-induced cytotoxicity after the pretreatments of resveratrol, rutin, fenugreek extract, blank nanoparticles (liponiosome without fenugreek extract), LNF nanoparticles, and 0.005% DMSO as a control (vehicle). HaCAT and human dermal fibroblast cells were co-cultured and pretreated with 7 µg/mL of extract and 100 µg/mL of LNF (7 µg/mL of extract equivalence), together with 100 µg/mL of blank, 10 µg/mL of resveratrol, 7 µg/mL of rutin as a positive, and 0.005% of DMSO as a control (vehicle), for 24 h before UV exposure. Data are means ± SD ( n = 3). *, p < 0.05. ( b ) The levels of UV-induced MMP1 and MMP9 secretions after fenugreek extract and LNF treatments. Data are reported as means ± SD ( n = 3). *, p < 0.05. ( c ) The levels of UV-induced IL-6 and IL-8 secretions after fenugreek extract and LNF treatments. Data are reported as means ± SD ( n = 3). *, p < 0.05.

Article Snippet: Human dermal fibroblast cells were purchased from the American Type Culture Collection: ATCC (PCS-201-010) and immortalized human keratinocytes (HaCaT) were purchased from Cell Lines Service, Germany (Cat. No. 300493).

Techniques: Inhibition, Cell Culture, Control